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Thermofluor

Step-by-step guide on how to use the Excel VBA macro to process Thermofluor data collected on the Applied Biosystems StepOnePlus rtPCR machine

First, you can download the VBA code by clicking HERE
Mac USERS, you will need the older version HERE

1) Open up the Excel "multicomponent data" spreadsheet exported by the rtPCR machine

2) In Excel, click on the "Tools" dropdown

3) Select "Macro", and then select "Visual Basic Editor"

4) In the new window click on the "Insert" dropdown, and select "Module"

5) Cut and paste the VBA code into the new box.

6) To run the macro, just click on the PLAY icon towards the center of the top toolbar

Your processed data is in the new worksheet labeled "Processed Data"